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Capped mRNAs have been created using the mMessage mMachine system (Ambion). Morpholinos (Gene Instruments) along with mRNAs ended up inserted to the 1�C2 cellular period embryos. GST-Myc-tagged mouse button Inv (GST-mInv-Myc) and FLAG-tagged mouse Nek8 (FLAG-mNek8) manufactured in Escherichia coli BL21. Cellular material were lysed in 20?mM Hepes pH Several.2, 400?mM NaCl, 10?mM EDTA, 2?mM EGTA, p

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