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Crosses were made on minimal salt agar (MN) with sterile toothpicks placed on the agar surface as described #links# by Lombard et?al., 2010b?and?Lombard et?al., 2010d. Isolates were crossed with themselves as controls, thus making it possible to distinguish between heterothallic and homothallic mating systems of the isolates. The plates were stacked in plastic containers and incubated at 20?��C fo


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