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After the?medium has been collected, cells have been washed when with phosphate-buffered saline (PBS), lysed together with NP-40 load, and afflicted by about three freeze-thaw menstrual cycles. Both the culture method and the mobile lysates have been centrifuged from A dozen,000?rpm in 4��C for 10?min to pay off mobile dirt. Cell lysate supernatants have been assayed with regard to necessary prote

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