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The full quantity has been taken to 50?mL by building methanol. The assay impulse contained A single.0?mL with the methanol extract along with 3.5?mL associated with Folin�CCiocalteu reagent. Soon after 3?min, the response was ceased using 1.5?mL involving 20% sea salt carbonate and #links# held from room temperature regarding 2?h, protected from gentle. Your absorbance had been attained at 765?nm


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